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Stabilized filter-supported bilayer lipid membranes (BLMs) for automated flow monitoring of compounds of clinical, pharmaceutical, environmental and industrial interest

机译:稳定的过滤器支持的双层脂质膜(BLM)用于自动流动 监测具有临床,制药,环境和工业利益的化合物

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摘要

This paper describes the results of analytical applications of electrochemical biosensors based on bilayer lipid membranes (BLMs) for the automated rapid and sensitive flow monitoring of substrates of hydrolytic enzymes, antigens and triazine herbicides.BLMs, composed of mixtures of egg phosphatidylcholine (egg PC) and dipalmitoylphosphatidic acid (DPPA), were supported on ultrafiltration membranes (glass microfibre or polycarbonate filters) which were found to enhance their stability for flow experiments. The proteins (enzymes, antibodies) were incorporated into a floating lipid matrix at an air-electrolyte interface, and then a casting procedure was used to deliver thelipid onto the filter supports for BLM formation. Injections of the analyte were made into flowing streams of the carrier electrolyte solution and a current transient signal was obtained with a magnitude related to the analyte concentration. Substrates of hydrolytic enzyme reactions (acetylcholine, urea and penicillin) could be determined at the micromolar level with a maximum rate of 220 samples/h, whereas antigens (thyroxin) and triazine herbicides (simazine, atrazine and propazine) could be monitored at the nanomolar level in less than 2 min. The time of appearance of the transient response obtained for herbicides was increased to the order of simazine, atrazine and propazine which has permitted analysis of these triazines in mixtures.
机译:本文介绍了基于双层脂质膜(BLM)的电化学生物传感器在水解酶,抗原和三嗪除草剂底物的自动化快速灵敏流动监测中的分析应用结果.BLM由卵磷脂酰胆碱(egg PC)的混合物组成超滤膜(玻璃微纤维或聚碳酸酯滤膜)上载有磷酸二棕榈酯和二棕榈酰磷脂酸(DPPA),发现该膜可增强其在流动实验中的稳定性。将蛋白质(酶,抗体)在空气-电解质界面处掺入浮动脂质基质中,然后使用流延程序将脂质递送到滤膜支持物上以形成BLM。将分析物注入到载体电解质溶液的流动物流中,并获得具有与分析物浓度相关的幅度的电流瞬变信号。可在微摩尔水平上测定水解酶反应(乙酰胆碱,尿素和青霉素)的底物,最大速率为220样本/小时,而抗原(甲状腺素)和三嗪除草剂(西玛津,阿特拉津和丙嗪)可在纳摩尔级进行监测不到2分钟的时间。除草剂获得的瞬态响应出现的时间增加到了simazine,atrazine和propazine的数量级,从而可以分析混合物中的这些三嗪。

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